Nitrate-responsive NarX-NarL represses arginine-mediated induction of the Pseudomonas aeruginosa arginine fermentation arcDABC operon
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چکیده
منابع مشابه
Nitrate-responsive NarX-NarL represses arginine-mediated induction of the Pseudomonas aeruginosa arginine fermentation arcDABC operon.
Denitrification and arginine fermentation are major parts of the anaerobic metabolism of Pseudomonas aeruginosa, which is important for biofilm formation and infection. The two-component regulatory system NarX-NarL is part of the underlying network and is required for denitrifying growth. All target promoters identified so far are activated by NarL. In this study the effect of NarL on arginine ...
متن کاملarcD, the first gene of the arc operon for anaerobic arginine catabolism in Pseudomonas aeruginosa, encodes an arginine-ornithine exchanger.
In the absence of oxygen and nitrate, Pseudomonas aeruginosa metabolizes arginine via the arginine deiminase pathway, which allows slow growth on rich media. The conversion of arginine to ornithine, CO2, and NH3 is coupled to the production of ATP from ADP. The enzymes of the arginine deiminase pathway are organized in the arcDABC operon. The arcD gene encodes a hydrophobic polytopic membrane p...
متن کاملControl of arginine biosynthesis in Pseudomonas aeruginosa.
Arginine biosynthesis in Pseudoinonas aeruginosa proceeded via transacetylation of acetylornithine with glutamate ; it resembled Micrococcus glutamicus rather than Escherichia coli. Of four arginine biosynthetic enzymes, N-acetyl-y-glutamokinase, N-acetylornithine glutamate transacetylase, ornithine transcarbamylase and argininosuccinase determined under various conditions of arginine excess an...
متن کاملHierarchical control of anaerobic gene expression in Escherichia coli K-12: the nitrate-responsive NarX-NarL regulatory system represses synthesis of the fumarate-responsive DcuS-DcuR regulatory system.
Hierarchical control ensures that facultative bacteria preferentially use the available respiratory electron acceptor with the most positive standard redox potential. Thus, nitrate is used before other electron acceptors such as fumarate for anaerobic respiration. Nitrate regulation is mediated by the NarX-NarL two-component system, which activates the transcription of operons encoding nitrate ...
متن کاملAnaerobically controlled expression system derived from the arcDABC operon of Pseudomonas aeruginosa: application to lipase production.
The anaerobically inducible arcDABC operon encodes the enzymes of the arginine deiminase pathway in Pseudomonas aeruginosa. Upon induction, the arcAB mRNAs and proteins reach high intracellular levels, because of a strong anaerobically controlled promoter and mRNA processing in arcD, leading to stable downstream transcripts. We explored the usefulness of this system for the construction of expr...
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ژورنال
عنوان ژورنال: Microbiology
سال: 2008
ISSN: 1350-0872,1465-2080
DOI: 10.1099/mic.0.2008/018929-0